Reporter

Part:BBa_K176114:Design

Designed by: Danqian Liu, Hao Jiang   Group: iGEM09_USTC   (2009-09-07)


CI Inverter + GFP Reporter: VR5+pCon 0.01->cI-LVA+pCI->GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 35
    Illegal NheI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1728


Design Notes

Input: pCon 0.01

Output: GFP

Design:

pCon 0.01 generates CI-LVA

pCI generates Output GFP


Source

K176105 K176107

References